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Methods

• Genetic engineering of bacteria and cyanobacteria (cloning, transformation, recombination etc.)

• Purification of recombinant proteins

• qPCR for the quantitative analysis of gene expression

• PAM fluorymetry in the monitoring of the light phase of photosynthesis in cyanobacteria

• Cyanophage titration by MPN and qPCR

• Determination of  the activity of selected enzymes of cyanobacteria, lichens and plants by colorimetric and fluorometric methods 

• The isolation of cyanobacterial membranes by centrifugation in saccharose gradient

• Determination of membrane fluidity by EPR

• Measurement of the intensity of respiration and photosynthesis processes in plants, algae and cyanobacteria using the Clark oxygen electrode and an infrared gas analyzer (IRGA)

• Analysis of the concentration of selected primary and secondary metabolites by HPLC

• Determination of photosynthetic pigments concentration by colorimetric method

• Purification and concentration of selected secondary metabolites using Solid Phase Extraction (SPE)

• Colorimetric measurement of lipid peroxidation